Before Beast Boy joined the Teen Titans, he was a member of the Doom Patrol, the heroic enemies of the Brotherhood of Evil. The Doom Patrol constantly foils the Brotherhood's schemes, but the Brotherhood always escapes, vowing revenge. Not much is known about the rivalry of the two groups after Beast Boy quit the team and joined the Titans.In Idol Hands, The Brotherhood of Evil appear at the Jump City's Got Talent show, competing against the Teen Titans. For their performances, The Brain planned to sing an A cappella medley of Lady Gaga songs, while the rest of his team performed interpretive dance. The Titans realize that if the Brotherhood performed, they would certainly win the show, so the Titans attempt to stop the villains before their performance begins. The villains give up on their performance and try to kill the Titans. After a fight described as a "Titanic, Earth-shattering battle", the Titans defeat the villains, only to have their fighting styles ridiculed by the judges.
- Brian (The Brain and Monsieur Mallah only)
- Campfire Stories (The Brain only, cameo)
- Some of Their Parts (Plasmus only)
- I'm the Sauce (The Brain, Monsieur Mallah and Plasmus only)
- 40%, 40%, 20% (The Brain and Monsieur Mallah only)
- A Farce (The Brain and Monsieur Mallah only)
- Black Friday (The Brain only)
- Operation Dude Rescue (The Brain only)
- The Titans Show (The Brain, Monsieur Mallah, and Plasmus only)
- The Cape (Plasmus only)
- Shrimps and Prime Rib (The Brain and Monsieur Mallah only)
- The Streak (The Brain and Monsieur Mallah only)
- Costume Contest (The Brain and Plasmus only)
- Beast Girl (Madame Rouge only)
- Flashback (Monsieur Mallah and Plasmus only)
Appearances in other Media
- Teeny Titans (The Brain and Monsieur Mallah only)
- Slash of Justice (The Brain and Monsieur Mallah only)
- In the original series, the Brotherhood of Evil served as the main antagonists of the show's final season.
- In the original series, Plasmus and Phobia were not part of the group, and General Immortus was. However, Immortus does make a brief cameo at the beginning of the comic the team debuted in.
- In the original series, almost all of the villains from previous seasons joined the Brotherhood.